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IHCPlus™ TNFSF9 / CD137L Antibody Polyclonal ELISA,IHC,WB LS‑B7425

Catalog Number Size Price
LS-B7425-50 50 µg $425 
Anti-TNFSF9 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ TNFSF9 / CD137L Antibody Polyclonal ELISA,IHC,WB LS‑B7425

Note: This antibody replaces LS-C104307, LS-C187796
Antibody:
Rabbit Polyclonal to Human TNFSF9 / CD137L
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B7425-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TNFSF9 / CD137L
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD137L antibody LS-B7425 is an unconjugated rabbit polyclonal antibody to human CD137L (TNFSF9 ). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TNFSF9 / CD137L
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E.coli derived recombinant Human 4-1BBL.
Specificity
Human 4-1BBL
Usage
Immunohistochemistry: LS-B7425 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: Data Not Available. ELISA: To detect h4-1BBL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human 4-1BBL (LS-C104681) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant h4-1BBL. Western Blot: To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant h4-1BBL is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFSF9 / CD137L
P41273 NM_003811 NP_003802.1

Validation

Anti-TNFSF9 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-TNFSF9 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TNFSF9 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/22/2019