IHC‑plus™ Monoclonal Mouse anti‑Human TNFRSF11A / RANK Antibody (clone 9A725, aa326‑616, IHC, WB) LS‑B2076
IHC‑plus™ Monoclonal Mouse anti‑Human TNFRSF11A / RANK Antibody (clone 9A725, aa326‑616, IHC, WB) LS‑B2076
Note: This antibody replaces LS-C591, LS-C93632, LS-C17581
Antibody:
TNFRSF11A / RANK Mouse anti-Human Monoclonal (aa326-616) (9A725) Antibody
Application:
IHC, IHC-P, WB, Flo
Format:
Unconjugated, Unmodified
Overview
Antibody:
TNFRSF11A / RANK Mouse anti-Human Monoclonal (aa326-616) (9A725) Antibody
Application:
IHC, IHC-P, WB, Flo
Format:
Unconjugated, Unmodified
Specifications
Description
RANK antibody LS-B2076 is an unconjugated mouse monoclonal antibody to RANK (TNFRSF11A) (aa326-616) from human. It is reactive with human and mouse. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TNFRSF11A / RANK
Synonyms
TNFRSF11A | CD265 | CD265 antigen | ODFR | LOH18CR1 | OPTB7 | OSTS | PDB2 | RANK | Receptor activator of NF-KB | TRANCER | FEO | OFE
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
A fusion protein containing amino acid residues 326-616 of human RANK.
Specificity
In RAW cells, a 97 kD is observed.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2076 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2076 was determined to be 10 ug/ml.
Presentation
PBS containing 0.5 % BSA, 0.05% sodium azide.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Anti-TNFRSF11A / RANK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TNFRSF11A / RANK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
Flow Cytometry
Species:
Human
Applications:
Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot
Reactivity:
Mouse
Range:
625-5000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/7/2024