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Catalog Number Size Price
LS-B2076-50 50 µg (1 mg/ml) $460 
TNFRSF11A / RANK Antibody - Anti-TNFRSF11A / RANK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
TNFRSF11A / RANK Antibody - Western blot of RANK in 293 cells transfected with RANK cDNA. A single protein band with an approximate mol. wt. of 97 kDa is detected.
TNFRSF11A / RANK Antibody - Flow analysis of RANK in 10^6 RAW 264.7 cells using 2 ug of antibody.  Shaded histogram represents RAW cells without antibody; green represents isotype control; red represents anti-RANK antibody.
TNFRSF11A / RANK Antibody - Anti-TNFRSF11A / RANK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
TNFRSF11A / RANK Antibody - Western blot of RANK in 293 cells transfected with RANK cDNA. A single protein band with an approximate mol. wt. of 97 kDa is detected.
TNFRSF11A / RANK Antibody - Flow analysis of RANK in 10^6 RAW 264.7 cells using 2 ug of antibody.  Shaded histogram represents RAW cells without antibody; green represents isotype control; red represents anti-RANK antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human TNFRSF11A / RANK Antibody (clone 9A725, aa326‑616, IHC, WB) LS‑B2076

IHC‑plus™ Monoclonal Mouse anti‑Human TNFRSF11A / RANK Antibody (clone 9A725, aa326‑616, IHC, WB) LS‑B2076

Note: This antibody replaces LS-C591, LS-C93632, LS-C17581
Antibody:
TNFRSF11A / RANK Mouse anti-Human Monoclonal (aa326-616) (9A725) Antibody
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2076-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TNFRSF11A / RANK Mouse anti-Human Monoclonal (aa326-616) (9A725) Antibody
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RANK antibody LS-B2076 is an unconjugated mouse monoclonal antibody to RANK (TNFRSF11A) (aa326-616) from human. It is reactive with human and mouse. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TNFRSF11A / RANK
Synonyms
TNFRSF11A | CD265 | CD265 antigen | ODFR | LOH18CR1 | OPTB7 | OSTS | PDB2 | RANK | Receptor activator of NF-KB | TRANCER | FEO | OFE
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
9A725
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A fusion protein containing amino acid residues 326-616 of human RANK.
Epitope
aa326-616
Specificity
In RAW cells, a 97 kD is observed.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2076 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2076 was determined to be 10 ug/ml.
Presentation
PBS containing 0.5 % BSA, 0.05% sodium azide.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFRSF11A / RANK
Q9Y6Q6 NM_003839 NP_003830.1

Validation

TNFRSF11A / RANK Antibody - Anti-TNFRSF11A / RANK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TNFRSF11A / RANK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TNFRSF11A / RANK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ TNFRSF11A / RANK Antibody (aa326-616, clone 9A725) for IHC, WB/Western, Flow LS-B2076 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/7/2024