Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-B9774-300 | 300 µg (3 mg/ml) | $460 |
1 of 6
2 of 6
3 of 6
4 of 6
5 of 6
6 of 6
PathPlus™ Polyclonal Goat anti‑Human TJP1 / ZO‑1 Antibody (C‑Terminus, IHC, IF, WB) LS‑B9774
PathPlus™ Polyclonal Goat anti‑Human TJP1 / ZO‑1 Antibody (C‑Terminus, IHC, IF, WB) LS‑B9774
Note: This antibody replaces LS-C204268
Antibody:
TJP1 / ZO-1 Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
TJP1 / ZO-1 Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Specifications
Description
TJP1 (Tight junction protein-1, Zonula occludens-1, ZO-1) is a scaffold protein involved in tight junction assembly, anchoring and stabilization. It functions in cell-cell junction signal transduction and in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. TJP1 is upregulated in and drug-resistant multiple myeloma, and is associated with a higher risk subset of cancers. TJP1 has been found to be upregulated in drug-resistant tumors. In immunohistochemistry of normal tissue, TJP1 has membranous positivity in endothelial cells, glandular epithelia, renal glomeruli, sertoli cells, and is expressed in most tissues throughout the body.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; Oncoscience. 2017 Jul; 4(7-8): 79–94, PMID: 28966941
Target
Human TJP1 / ZO-1
Synonyms
TJP1 | ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | Zonula occludens 1 protein
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Purified recombinant peptide derived from within residues 1580 aa to the C-Terminus of human ZO-1 produced in E. coli.
Epitope
C-Terminus
Specificity
Detects endogenous levels of total ZO-1 by Western blot in whole cell and tissue lysates.
Applications
- IHC
- IHC - Paraffin (5 - 10 µg/ml)
- IHC - Frozen (1:50 - 1:200)
- Immunofluorescence (1:50 - 1:250)
- Western blot (1:500 - 1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Presentation
PBS, 0.05% Sodium Azide, 20% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TJP1 / ZO-1
Validation
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin, Stratum Basale: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin, Stratum Basale: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney, Positive Signal in Collecting Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney, Positive Signal in Collecting Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis, Positive signal in Sertoli Cell Membrane: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis, Positive signal in Sertoli Cell Membrane: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ TJP1 / ZO-1 Antibody (C-Terminus) for IHC, IF/Immunofluorescence, WB/Western LS-B9774 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (3)
Inhibition of Retinal Ganglion Cell Loss By a Novel ROCK Inhibitor (E212) in Ischemic Optic Nerve Injury Via Antioxidative and Anti-Inflammatory Actions. Yao-Tseng Wen, Ching-Wen Huang, Chih-Peng Liu, Chih-Hung Chen, Chia-Mu Tu, Chrong-Shiong Hwang, Yi-Hsun Chen, Wan-Ru Chen, Keh-Liang Lin, Yu-Chieh Ho, Ta-Ching Chen , Rong-Kung Tsai. The Breast : official journal of the European Society of Mastology. 2021 May;62:21.
AMPK modulation ameliorates dominant disease phenotypes of CTRP5 variant in retinal degeneration. Kiyoharu J Miyagishima , Ruchi Sharma , Malika Nimmagadda , Katharina Clore-Gronenborn, Zoya Qureshy, Davide Ortolan, Devika Bose, Mitra Farnoodian, Congxiao Zhang, Andrew Fausey, Yuri V Sergeev, Mones Abu-Asab, Bokkyoo Jun, Khanh V Do, Marie-Audrey Kautzman Guerin, Jorgelina Calandria, Aman George, Bin Guan, Qin Wan, Rachel C Sharp, Catherine Cukras, Paul A Sieving, Robert B Hufnagel, Nicolas G Bazan, Kathleen Boesze-Battaglia, Sheldon Miller, Kapil Bharti. Communications biology. 2021 December;4:1360.
Induction of Human Umbilical Mesenchymal Stem Cell Differentiation Into Retinal Pigment Epithelial Cells Using a Transwell-Based Co-culture System. Yu-Hsun Chang, V Bharath Kumar, Yao-Tseng Wen, Chih-Yang Huang, Rong-Kung Tsai, Dah-Ching Ding. Cell transplantation. 2022 March;31:9636897221085901.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human, Mouse, Rat, Dog, Guinea pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
