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Catalog Number Size Price
LS-B347-50 50 µl $515 
TIP120 / CAND1 Antibody - Anti-CAND1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC‑plus™ Polyclonal Rabbit anti‑Human TIP120 / CAND1 Antibody (aa11‑24, IHC, WB) LS‑B347

IHC‑plus™ Polyclonal Rabbit anti‑Human TIP120 / CAND1 Antibody (aa11‑24, IHC, WB) LS‑B347

Note: This antibody replaces LS-C18987
Antibody:
TIP120 / CAND1 Rabbit anti-Human Polyclonal (aa11-24) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B347-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TIP120 / CAND1 Rabbit anti-Human Polyclonal (aa11-24) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CAND1 antibody LS-B347 is an unconjugated rabbit polyclonal antibody to human CAND1 (TIP120) (aa11-24). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human TIP120 / CAND1
Synonyms
CAND1 | KIAA0829 | TBP interacting protein | TBP-interacting protein 120A | TIP120 | p120 CAND1 | TIP120A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated followed by sterile filtration
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH.
Epitope
aa11-24
Specificity
Reacts with human, rat and mouse CAND1/TIP120A. Cross reactivity does occur with human, rat and mouse CAND2/TIP120B. Cross reactivity with CAND1 from other sources is not known.
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B347 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B347 was determined to be 1:500.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TIP120 / CAND1
Q86VP6 NM_018448 NP_060918.2

Validation

TIP120 / CAND1 Antibody - Anti-CAND1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-CAND1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-CAND1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

LSBio Ratings

IHC-plus™ TIP120 / CAND1 Antibody (aa11-24) for IHC, WB/Western, IP, ELISA LS-B347 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)

Publications (3)

NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Liu J, Furukawa M, Matsumoto T, Xiong Y. Molecular cell. 2002 10:1511-8.

AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis. Cheng Y, Dai X, Zhao Y. Plant physiology. 2004 135:1020-6.

Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. Feng S, Shen Y, Sullivan JA, Rubio V, Xiong Y, Sun TP, Deng XW. The Plant cell. 2004 16:1870-82.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/25/2024