Immunohistochemistry: LS-B41 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B41 was determined to be 1-2.5 ug/ml. Flow Cytometry: Membrane permeabilization is required for this application. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. Histology Positive Control Tissue: Human Breast Carcinoma.
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.