Human TG / Thyroglobulin

Mouse

Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

2H11

Unconjugated

Protein A purified

Unmodified

This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein.

Reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity with thyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has not been determined.

• IHC
• IHC - Paraffin (5 µg/ml)
• IHC - Frozen (1:50 - 1:200)
• Western blot (1:50 - 1:200)
• ELISA (1:20000 - 1:100000)

Immunohistochemistry: LS-B428 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B428 was determined to be 5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01266 NCBI: NM_003235 NP_003226.4