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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B427-50 50 µg (1 mg/ml) $485 
TFAP2A / AP-2 Antibody - Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TFAP2A / AP-2 Antibody - Anti-AP2A Antibody - Western Blot. Western blot of Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kD corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.
TFAP2A / AP-2 Antibody - Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TFAP2A / AP-2 Antibody - Anti-AP2A Antibody - Western Blot. Western blot of Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kD corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.
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IHCPlus™ Polyclonal TFAP2A / AP‑2 Antibody (aa1‑25, IHC, WB) LS‑B427

IHCPlus™ Polyclonal TFAP2A / AP‑2 Antibody (aa1‑25, IHC, WB) LS‑B427

Note: This antibody replaces LS-C18899
Antibody:
Goat Polyclonal to Human TFAP2A / AP-2
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Chimpanzee, Orangutan, Mouse, Rat, Dog, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B427-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Human TFAP2A / AP-2
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Chimpanzee, Orangutan, Mouse, Rat, Dog, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
AP-2 antibody LS-B427 is an unconjugated goat polyclonal antibody to AP-2 (TFAP2A) (aa1-25) from human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TFAP2A / AP-2
Synonyms
TFAP2A | Activator protein 2 | AP-2 | AP-2a | AP2TF | AP-2alpha | BOFS | TFAP2 | Transcription factor AP-2 | AP-2 transcription factor | AP2-alpha
Host
Goat
Reactivity
Human, Chimpanzee, Orangutan, Mouse, Rat, Dog, Chicken (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Orangutan, Mouse, Rat, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of Human AP2A1 and AP2A2 proteins.
Epitope
aa1-25
Specificity
A BLAST analysis was used to suggest reactivity with AP2A1 and AP2A2 protein from human, chimpanzee, orangutan, mouse, rat, chicken and dog based on 100% homology for the immunogen sequence. Cross reactivity with AP2A proteins from other sources is not known.
Usage
Immunohistochemistry: LS-B427 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B427 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TFAP2A / AP-2
P05549 NM_003220 NP_003211.1

Validation

TFAP2A / AP-2 Antibody - Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ TFAP2A / AP-2 Antibody (aa1-25) for IHC, WB/Western, IP, ELISA LS-B427 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 4/6/2020