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IHCPlus™ TFAP2A / AP‑2 Antibody aa3‑14 Polyclonal ELISA,IF,IHC,WB LS‑B427

Catalog Number Size Price
LS-B427-50 50 µg (1 mg/ml) $485 
Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AP2A Antibody - Western Blot. Western blot of Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kD corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.
Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AP2A Antibody - Western Blot. Western blot of Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kD corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.
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IHCPlus™ TFAP2A / AP‑2 Antibody aa3‑14 Polyclonal ELISA,IF,IHC,WB LS‑B427

Note: This antibody replaces LS-C18899
Antibody:
Goat Polyclonal to Human TFAP2A / AP-2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B427-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human TFAP2A / AP-2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
AP-2 antibody LS-B427 is an unconjugated goat polyclonal antibody to AP-2 (TFAP2A ) from human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TFAP2A / AP-2
Host
Goat
Reactivity
Human, Mouse, Rat, Dog, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human TFAP2A / AP2 Alpha.
Epitope
aa3-14
Specificity
aa 3-14 of Human AP2A1 and AP2A2 proteins.
Usage
Immunohistochemistry: LS-B427 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B427 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TFAP2A / AP-2
P05549 NM_003220 NP_003211.1

Validation

Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TFAP2A / AP2 Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/17/2019