Human TF / Transferrin

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Ion exchange chromatography

Unmodified

Transferrin (Human Serum)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum, purified and partially purified transferrin (human serum). Cross-reactivity against transferrin from other tissues and species may occur by have not been tested.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot
• Immunoprecipitation
• ELISA (1:40000 - 1:160000)

Immunohistochemistry: LS-B2447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2447 was determined to be 5 ug/ml. ELISA: 1:40000-1:160000 (Coat with 1 ug transferrin in a standard sandwich ELISA using peroxidase conjugated rabbit IgG and ABTS as a substrate for 30 min at RT).

PBS, pH 7.2, 0.01% Sodium Azide, 40% Glycerol

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02787 NCBI: NM_001063 NP_001054.1