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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3586-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human TDP1 Antibody (clone AT1F2, IHC, WB) LS‑B3586
IHC‑plus™ Monoclonal Mouse anti‑Human TDP1 Antibody (clone AT1F2, IHC, WB) LS‑B3586
Note: This antibody replaces LS-C103493
Antibody:
TDP1 Mouse anti-Human Monoclonal (AT1F2) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
TDP1 Mouse anti-Human Monoclonal (AT1F2) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
TDP1 antibody LS-B3586 is an unconjugated mouse monoclonal antibody to human TDP1. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Clone
AT1F2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human TDP1 (1-298aa) purified from E. coli
Specificity
human TDP1 (1-298aa)
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000 - 1:2000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3586 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3586 was determined to be 5 ug/ml. Western Blot: Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TDP1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TDP1
Validation
Anti-TDP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TDP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TDP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TDP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ TDP1 Antibody (clone AT1F2) for IHC, WB/Western, ELISA LS-B3586 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
