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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-B5712-100 100 µl $485 
SRSF6 / SRP55 Antibody - Anti-SRSF6 / SFRS6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SRSF6 / SRP55 Antibody - Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SRSF6 / SRP55 Antibody - Anti-SRSF6 / SFRS6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SRSF6 / SRP55 Antibody - Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ Polyclonal Rabbit anti‑Human SRSF6 / SRP55 Antibody (aa143‑192, IHC, WB) LS‑B5712

IHCPlus™ Polyclonal Rabbit anti‑Human SRSF6 / SRP55 Antibody (aa143‑192, IHC, WB) LS‑B5712

Note: This antibody replaces LS-C80358
Antibody:
Rabbit Polyclonal to Human SRSF6 / SRP55
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig, Rabbit, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5712-100
100 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human SRSF6 / SRP55
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig, Rabbit, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
SRP55 antibody LS-B5712 is an unconjugated rabbit polyclonal antibody to SRP55 (SRSF6) (aa143-192) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human SRSF6 / SRP55
Synonyms
SRSF6 | B52 | Pre-mRNA-splicing factor SRP55 | SFRS6 | SR splicing factor 6 | SRP55
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa143-192 of human SRSF6 (Q13247, NP_006266). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Pig, Opossum (100%); Turkey, Zebra finch, Chicken, Platypus (85%); Stickleback, Zebrafish (84%).
Blocking Peptide
LS-E14865 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5712. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa143-192
Specificity
Human SRSF6 / SFRS6
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SRSF6 / SRP55
Q13247 NM_006275 NP_006266.2

Validation

SRSF6 / SRP55 Antibody - Anti-SRSF6 / SFRS6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SRSF6 / SFRS6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SRSF6 / SFRS6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ SRSF6 / SRP55 Antibody (aa143-192) for IHC, WB/Western LS-B5712 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

Aberrant splicing of HTT generates the pathogenic exon 1 protein in Huntington disease. Sathasivam K, Neueder A, Gipson TA, Landles C, Benjamin AC, Bondulich MK, Smith DL, Faull RL, Roos RA, Howland D, Detloff PJ, Housman DE, Bates GP. Proceedings of the National Academy of Sciences of the United States of America. 2013 110:2366-70. (IP; Mouse)

Huntington's disease is a four-repeat tauopathy with tau nuclear rods. Fern[Character e1]ndez-Nogales M, Cabrera JR, Santos-Galindo M, Hoozemans JJ, Ferrer I, Rozemuller AJ, Hern[Character e1]ndez F, Avila J, Lucas JJ. Nature medicine. Aug;20:881-5.

Customer Reviews (0)


Requested From: United States
Date Requested: 5/30/2020