Synthetic 15 amino acid peptide from N-terminus of human SPHK1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Gorilla, Dog, Bat, Elephant, Panda, Rabbit (93%); Horse (87%); Mouse, Rat, Bovine (80%).
Human SPHK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A3106
was determined to be 10 ug/ml.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.