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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B64-50 50 µg (1 mg/ml) $485 
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ Polyclonal Rabbit anti‑Human SMAD3 Antibody (phospho‑Ser423/425, IHC, WB) LS‑B64

IHCPlus™ Polyclonal Rabbit anti‑Human SMAD3 Antibody (phospho‑Ser423/425, IHC, WB) LS‑B64

Note: This antibody replaces LS-C18973, LS-C56911
Antibody:
Rabbit Polyclonal to Human SMAD3
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus laevis, Xenopus tropicalis
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B64-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human SMAD3
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus laevis, Xenopus tropicalis
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-B64 is an unconjugated rabbit polyclonal antibody to SMAD3 (pSer423/425) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SMAD3
Synonyms
SMAD3 | HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | HSMAD3 | Mad protein homolog | SMAD 3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus laevis, Xenopus tropicalis (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:2000 - 1:20000)
  • Flow Cytometry
  • ELISA (1:15000 - 1:30000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein.
Epitope
pSer423/425
Specificity
Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Usage
Immunohistochemistry: LS-B64 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B64 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

Validation

SMAD3 Antibody - Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ SMAD3 Antibody (phospho-Ser423/425) for IHC, WB/Western, Flow, ELISA LS-B64 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 6/1/2020