Human SLC2A4 / GLUT-4
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (10 - 20 µg/ml)
- IHC - Frozen
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A 12 amino acid peptide corresponding to the carboxyl terminus of the insulin regulatable glucose transporter conjugated to keyhole limpet hemocyanin.
Recognizes human Solute carrier family 2, facilitated glucose transporter member 4, also known as glucose transporter 4 or GLUT4. GLUT4 is a 509 amino acid ~50 kD multi-pass transmembrane protein located primarily in the perinuclear region and is translocated to the plasma membrane where it is rapidly reinternalized. Does not react with HEP G2 type glucose transporter in human erythrocytes, rat brain or other tissues that do not exhibit sensitivity to insulin. GLUT4 is the insulin-regulated glucose transporter found in adipose tissue and striated muscle, responsible for glucose disposal. The stimulation of glucose uptake by insulin requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.
Immunohistochemistry: LS-B26 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B26 was determined to be 10-20 ug/ml.
PBS, 0.09% Sodium Azide
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
For research use only.
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