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IHCPlus™ SLC2A4 / GLUT‑4 Antibody aa498‑509 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8335

Catalog Number Size Price
LS-B8335-50 50 µg (0.5 mg/ml) $425 
Anti-GLUT4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
SLC2A4 antibody (1 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-GLUT4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
SLC2A4 antibody (1 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ SLC2A4 / GLUT‑4 Antibody aa498‑509 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8335

Note: This antibody replaces LS-C139596
Antibody:
Goat Polyclonal to Human SLC2A4 / GLUT-4
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8335-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human SLC2A4 / GLUT-4
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
GLUT-4 antibody LS-B8335 is an unconjugated goat polyclonal antibody to GLUT-4 (SLC2A4 ) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SLC2A4 / GLUT-4
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-TELEYLGPDEND, from the C Terminus of the protein sequence according to NP_001033.1.
Blocking Peptide
LS-E27403 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8335. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa498-509
Specificity
Human SLC2A4 / GLUT4.
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 45kD band observed in Mouse and Rat Heart lysates (calculated MW of 54.8kD according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fischer et al, J Biol Chem. 1997 Mar 14;272(11):7085-92.; PMID: 9054401). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC2A4 / GLUT-4
P14672 NM_001042 NP_001033.1

Validation

Anti-GLUT4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IHC Validated
Anti-GLUT4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-GLUT4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Featured Products

Product
Species: Human
Applications: IHC, Western blot, ELISA
Anti-SLC2A4 / GLUT4 antibody IHC of human heart, cardiac myocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
SLC2A4 / GLUT4 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit
Applications: Western blot
HeLa cells stained with Mouse anti-Glucose Transporter 4:FITC and counterstained with DAPI This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence
IHC of paraffin-embedded bladder cancer tissues using SLC2A4 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Reactivity: Human
Range: 1.25-20 ng/ml

Requested From: United States
Date Requested: 8/23/2019
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