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IHCPlus™ SLC24A1 / NCKX Antibody aa612‑661 Polyclonal IHC,WB LS‑B9700

Catalog Number Size Price
LS-B9700-100 100 µl $485 
Anti-SLC24A1 / NCKX antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SLC24A1 antibody Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SLC24A1 / NCKX antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SLC24A1 antibody Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ SLC24A1 / NCKX Antibody aa612‑661 Polyclonal IHC,WB LS‑B9700

Note: This antibody replaces LS-C102060
Antibody:
Rabbit Polyclonal to Human SLC24A1 / NCKX
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B9700-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SLC24A1 / NCKX
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NCKX antibody LS-B9700 is an unconjugated rabbit polyclonal antibody to NCKX (SLC24A1 ) from human, rat and dog. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SLC24A1 / NCKX
Host
Rabbit
Reactivity
Human, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa612-661 of human SLC24A1 (O60721, NP_004718). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey, Galago (92%); Rat (84%).
Epitope
aa612-661
Specificity
Human SLC24A1
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC24A1 / NCKX
O60721 NM_004727 NP_004718.1

Validation

Anti-SLC24A1 / NCKX antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-SLC24A1 / NCKX antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SLC24A1 / NCKX antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Immunofluorescent analysis of NCKX1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Species: Human
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Requested From: United States
Date Requested: 9/19/2019