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Catalog Number Size Price
LS-B5941-50 50 µg (0.5 mg/ml) $485 
SLC12A2 / NKCC1 Antibody - Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
SLC12A2 / NKCC1 Antibody - Goat Anti-SLC12A2 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
SLC12A2 / NKCC1 Antibody - Goat Anti-SLC12A2 Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
SLC12A2 / NKCC1 Antibody - Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
SLC12A2 / NKCC1 Antibody - Goat Anti-SLC12A2 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
SLC12A2 / NKCC1 Antibody - Goat Anti-SLC12A2 Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human SLC12A2 / NKCC1 Antibody (aa769‑783, IHC, IF) LS‑B5941

IHC‑plus™ Polyclonal Goat anti‑Human SLC12A2 / NKCC1 Antibody (aa769‑783, IHC, IF) LS‑B5941

Note: This antibody replaces LS-C112911
Antibody:
SLC12A2 / NKCC1 Goat anti-Human Polyclonal (aa769-783) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Bovine, Dog, Horse, Pig, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B5941-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SLC12A2 / NKCC1 Goat anti-Human Polyclonal (aa769-783) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Bovine, Dog, Horse, Pig, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
NKCC1 antibody LS-B5941 is an unconjugated goat polyclonal antibody to NKCC1 (SLC12A2) (aa769-783) from human. It is reactive with human, bovine, chicken and other species. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human SLC12A2 / NKCC1
Synonyms
SLC12A2 | Basolateral Na-K-Cl symporter | BSC2 | MBSC2 | NKCC1 | Sy-ns | BSC
Host
Goat
Reactivity
Human, Bovine, Dog, Horse, Pig, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-NALQHSIRLSGVEDH, from the internal region of the protein sequence according to NP_001037.1NP_001243390.1.
Epitope
aa769-783
Specificity
Human SLC12A2 / NKCC1.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 75kDa band in Human, Mouse and Rat Skeletal Muscle, Heart and Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131kDa according to NP_001037.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC12A2 / NKCC1
P55011 NM_001046 NP_001037.1

Validation

SLC12A2 / NKCC1 Antibody - Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

LSBio Ratings

IHC-plus™ SLC12A2 / NKCC1 Antibody (aa769-783) for IHC, IF/Immunofluorescence LS-B5941 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024