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IHCPlus™ SLC12A2 / NKCC1 Antibody aa769‑783 Polyclonal IF,IHC,Peptide‑ELISA LS‑B5941

Catalog Number Size Price
LS-B5941-50 50 µg (0.5 mg/ml) $425 
Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-SLC12A2 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Goat Anti-SLC12A2 Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-SLC12A2 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Goat Anti-SLC12A2 Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHCPlus™ SLC12A2 / NKCC1 Antibody aa769‑783 Polyclonal IF,IHC,Peptide‑ELISA LS‑B5941

Note: This antibody replaces LS-C112911
Antibody:
Goat Polyclonal to Human SLC12A2 / NKCC1
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Bovine, Dog, Horse, Pig, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B5941-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human SLC12A2 / NKCC1
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Bovine, Dog, Horse, Pig, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
NKCC1 antibody LS-B5941 is an unconjugated goat polyclonal antibody to NKCC1 (SLC12A2 ) from human, bovine, chicken and other species. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human SLC12A2 / NKCC1
Host
Goat
Reactivity
Human, Bovine, Dog, Horse, Pig, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-NALQHSIRLSGVEDH, from the internal region of the protein sequence according to NP_001037.1NP_001243390.1.
Blocking Peptide
LS-E27251 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5941. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa769-783
Specificity
Human SLC12A2 / NKCC1.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 75kDa band in Human, Mouse and Rat Skeletal Muscle, Heart and Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131kDa according to NP_001037.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC12A2 / NKCC1
P55011 NM_001046 NP_001037.1

Validation

Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
IHC Validated
Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

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Requested From: United States
Date Requested: 9/18/2019