Rat SERPINH1 / HSP47

Mouse

Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

M16.10A1

Unconjugated

Protein G purified

Unmodified

Native rat Hsp47 (Colligin) protein (full length)

Recognizes rat HSP47 at ~47kD. Species cross-reactivity: human, monkey, mouse, rabbit, hamster, guinea pig, bovine, canine, sheep, porcine and chicken (weak).

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot
• ELISA

Immunohistochemistry: LS-B3111 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3111 was determined to be 5 ug/ml. Positive control: HeLa Heat Shocked Cell Lysate.

PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P50454 NCBI: NM_001235 NP_001226.2