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Catalog Number Size Price
LS-B1454-50 50 µl (10 mg/ml) $460 
SERPINA1 / Alpha 1 Antitrypsin Antibody - Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha 1 anti-trypsin antibody-Fluorescent western blot. Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (LS-C59393, green); Alpha 1 anti-trypsin (LS-C59210, red); and Human IgG (LS-C59376, Blue) in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was washed 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Bio-Rad VersaDoc4000.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha-1anti-Trypsin Polyclonal Antibody-Western blot. Goat anti-Alpha-1anti-Trypsin antibody was used to detect Alpha-1anti-Trypsin under reducing conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the Bio-Rad VersaDoc System.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin (LS-C59210 lot 5842, red), Rabbit anti-Transferrin (LS-C59393 lot 3033), and Mouse-a-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha 1 anti-trypsin antibody-Fluorescent western blot. Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (LS-C59393, green); Alpha 1 anti-trypsin (LS-C59210, red); and Human IgG (LS-C59376, Blue) in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was washed 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Bio-Rad VersaDoc4000.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha-1anti-Trypsin Polyclonal Antibody-Western blot. Goat anti-Alpha-1anti-Trypsin antibody was used to detect Alpha-1anti-Trypsin under reducing conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the Bio-Rad VersaDoc System.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin (LS-C59210 lot 5842, red), Rabbit anti-Transferrin (LS-C59393 lot 3033), and Mouse-a-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.
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IHC‑plus™ Polyclonal Goat anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (IHC, IF, WB) LS‑B1454

IHC‑plus™ Polyclonal Goat anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (IHC, IF, WB) LS‑B1454

Note: This antibody replaces LS-C18949
Antibody:
SERPINA1 / Alpha 1 Antitrypsin Goat anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B1454-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
SERPINA1 / Alpha 1 Antitrypsin Goat anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha 1 Antitrypsin antibody LS-B1454 is an unconjugated goat polyclonal antibody to human Alpha 1 Antitrypsin (SERPINA1). Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SERPINA1 / Alpha 1 Antitrypsin
Synonyms
SERPINA1 | A1A | A1AT | AAT | Alpha 1 Antitrypsin | Alpha-1 protease inhibitor | Alpha-1-antiproteinase | Alpha-1-antitrypsin | Alpha1AT | PRO2275 | Alpha-1-antitrypsin null | PI1 | Serpin A1 | Alpha 1 AT
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Purified human SERPINA1 / Alpha-1-Antitrypsin.
Specificity
A1-Anti-Trypsin [Human Plasma].
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1454 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1454 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% (w/v) Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPINA1 / Alpha 1 Antitrypsin
P01009 K02212 AAB59495.1

Validation

SERPINA1 / Alpha 1 Antitrypsin Antibody - Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ SERPINA1 / Alpha 1 Antitrypsin Antibody for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B1454 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/16/2021