IHC‑plus™ Monoclonal Mouse anti‑Human SAFB1 / SAFB Antibody (clone 6F7, aa345‑357, IHC, IF, WB) LS‑B1712
IHC‑plus™ Monoclonal Mouse anti‑Human SAFB1 / SAFB Antibody (clone 6F7, aa345‑357, IHC, IF, WB) LS‑B1712
Note: This antibody replaces LS-C20007
Antibody:
SAFB1 / SAFB Mouse anti-Human Monoclonal (aa345-357) (6F7) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Overview
Antibody:
SAFB1 / SAFB Mouse anti-Human Monoclonal (aa345-357) (6F7) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
SAFB antibody LS-B1712 is an unconjugated mouse monoclonal antibody to SAFB (SAFB1) (aa345-357) from human. It is reactive with human and monkey. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SAFB1 / SAFB
Synonyms
SAFB | HAP | HET | Scaffold attachment factor B | SAB-B1 | SAFB1 | SAF-B | SAF-B1 | Scaffold attachment factor B1 | Hsp27 ERE-TATA binding protein | HSP27 ERE-TATA-binding protein
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Immunogen
A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human SAFB protein.
Specificity
A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence
- Western blot
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1712 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1712 was determined to be 5 ug/ml.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Anti-SAFB antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SAFB antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.312-20 ng/ml
Reactivity:
Mouse
Range:
0.78-50 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/28/2024