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Catalog Number Size Price
LS-B10465-50 50 µg (0.5 mg/ml) $485 
RUFY1 Antibody - Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
RUFY1 Antibody - RUFY1 antibody (0.05 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
RUFY1 Antibody - Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
RUFY1 Antibody - RUFY1 antibody (0.05 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human RUFY1 Antibody (aa479‑491, IHC, WB) LS‑B10465

IHC‑plus™ Polyclonal Goat anti‑Human RUFY1 Antibody (aa479‑491, IHC, WB) LS‑B10465

Note: This antibody replaces LS-C139714
Antibody:
RUFY1 Goat anti-Human Polyclonal (aa479-491) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B10465-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RUFY1 Goat anti-Human Polyclonal (aa479-491) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
RUFY1 antibody LS-B10465 is an unconjugated goat polyclonal antibody to RUFY1 (aa479-491) from human. It is reactive with human, monkey and rabbit. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RUFY1
Synonyms
RUFY1 | FYVE-finger protein EIP1 | RABIP4 | La-binding protein 1 | Rab4-interacting protein | ZFYVE12
Host
Goat
Reactivity
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-QKNEAITSFEGKT, from the internal region of the protein sequence according to NP_079434.3NP_001035541.1.
Epitope
aa479-491
Specificity
Human RUFY1. This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.05 - 0.2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 85kD band observed in lysates of cell line HeLa (calculated MW of 79.8kD according to NP_079434.3). Recommended concentration: 0.05-0.2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RUFY1
Q96T51 NM_025158 NP_079434.3

Validation

RUFY1 Antibody - Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ RUFY1 Antibody (aa479-491) for IHC, WB/Western LS-B10465 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024