Human RNF2 / RING2 / RING1B

Goat

Human, Chimpanzee, Orangutan, Mouse, Rat, Bovine, Dog, Chicken, Frog (tested or 100% immunogen sequence identity)

Chimpanzee, Orangutan, Mouse, Rat, Bovine, Dog, Chicken, Frog, Zebrafish (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.

aa189-201

A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Immunofluorescence (1:300)
• Western blot (1:500 - 1:2000)
• ELISA (1:5000 - 1:25000)

Immunohistochemistry: LS-B417 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B417 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99496 NCBI: NM_007212 NP_009143.1