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IHCPlus™ RNF139 / TRC8 Antibody aa651‑663 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8293

Catalog Number Size Price
LS-B8293-50 50 µg (0.5 mg/ml) $425 
Anti-RNF139 / TRC8 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-TRC8 Antibody (1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
RNF139 antibody (0.5 ug/ml) staining of Human Skeletal muscle lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-RNF139 / TRC8 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-TRC8 Antibody (1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
RNF139 antibody (0.5 ug/ml) staining of Human Skeletal muscle lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ RNF139 / TRC8 Antibody aa651‑663 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8293

Note: This antibody replaces LS-C112821
Antibody:
Goat Polyclonal to Human RNF139 / TRC8
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8293-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human RNF139 / TRC8
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
TRC8 antibody LS-B8293 is an unconjugated goat polyclonal antibody to TRC8 (RNF139 ) from human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RNF139 / TRC8
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-HTGAAAEEFNDDT, from the C Terminus of the protein sequence according to NP_009149.2.
Blocking Peptide
LS-E25521 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8293. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa651-663
Specificity
Human RNF139.
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Experiments gave bands at approx. 150kDa and 50kDa in Human Skeletal Mucle lysate after 0.5 ug/ml antibody staining. This protein has a calculated MW of 76.0kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RNF139 / TRC8
Q8WU17 NM_007218 NP_009149.2

Validation

Anti-RNF139 / TRC8 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IHC Validated
Anti-RNF139 / TRC8 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-RNF139 / TRC8 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Featured Products

All lanes: Anti-RNF139 Antibody (C-Term) at 1:2000 dilution. Lane 1: Caki-1 whole cell lysate. Lane 2: human brain lysate. Lane 3: human heart lysate. Lane 4: human kidney lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity: Mouse
Range: 3.125-200 ng/ml
Reactivity: Chicken
Range: 78.125-5000 pg/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Mouse
Range: 6.17-500 pg/ml

Requested From: United States
Date Requested: 8/22/2019
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