Human RNF139 / TRC8
(tested or 100% immunogen sequence identity)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
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Peptide with sequence C-HTGAAAEEFNDDT, from the C Terminus of the protein sequence according to NP_009149.2.
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8293. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Experiments gave bands at approx. 150kDa and 50kDa in Human Skeletal Mucle lysate after 0.5 ug/ml antibody staining. This protein has a calculated MW of 76.0kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.