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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B218-100 100 µl $425 
RNASEL / RNase L Antibody - Anti-RNASEL / RNase L antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHCPlus™ Monoclonal RNASEL / RNase L Antibody (clone 2E9, IHC, WB) LS‑B218

IHCPlus™ Monoclonal RNASEL / RNase L Antibody (clone 2E9, IHC, WB) LS‑B218

Note: This antibody replaces LS-C2877
Antibody:
Mouse Monoclonal to Human RNASEL / RNase L
Application:
IHC, IHC-P, WB
Reactivity:
Human, Hamster, Primate
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B218-100
100 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human RNASEL / RNase L
Application:
IHC, IHC-P, WB
Reactivity:
Human, Hamster, Primate
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
RNase L antibody LS-B218 is an unconjugated mouse monoclonal antibody to RNase L (RNASEL) from human, hamster and primate. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RNASEL / RNase L
Synonyms
RNASEL | 2-5A-dependent ribonuclease | 2-5A-dependent RNAase | 2-5A-dependent RNase | HPC1 | PRCA1 | RNS4 | RNase L | Prostate cancer 1 | Ribonuclease 4 | Ribonuclease L
Host
Mouse
Reactivity
Human, Hamster, Primate (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1,k Monoclonal
Clone
2E9
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:1000 - 1:4000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified full-length recombinant human RNaseL produced in insect cells.
Specificity
human RNaseL.
Usage
Immunohistochemistry: LS-B218 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B218 was determined to be 2.5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RNASEL / RNase L
Q05823 NM_021133 NP_066956.1

Validation

RNASEL / RNase L Antibody - Anti-RNASEL / RNase L antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RNASEL / RNase L antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RNASEL / RNase L antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ RNASEL / RNase L Antibody (clone 2E9) for IHC, WB/Western LS-B218 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 3/30/2020