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IHCPlus™ RANGAP1 Antibody Monoclonal Flo,IF,IHC,WB LS‑B14772

Catalog Number Size Price
LS-B14772-100 100 µl (0.81 mg/ml) $425 
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC of paraffin-embedded Human prostate tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
Anti-RANGAP1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RANGAP1 antibody, and then analyzed by flow cytometry.
Flow cytometry of HeLa cells, using anti-RANGAP1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of Jurkat cells, using anti-RANGAP1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC of paraffin-embedded Human prostate tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-RANGAP1 mouse monoclonal antibody. (Dilution 1:50).
Anti-RANGAP1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RANGAP1 antibody, and then analyzed by flow cytometry.
Flow cytometry of HeLa cells, using anti-RANGAP1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of Jurkat cells, using anti-RANGAP1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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IHCPlus™ RANGAP1 Antibody Monoclonal Flo,IF,IHC,WB LS‑B14772

Note: This antibody replaces LS-C115280
Antibody:
Mouse Monoclonal to Human RANGAP1
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B14772-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human RANGAP1
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
RANGAP1 antibody LS-B14772 is an unconjugated mouse monoclonal antibody to RANGAP1 from human, rat and dog. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RANGAP1
Host
Mouse
Reactivity
Human, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
1B4
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50) & (5 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.
Specificity
Human RANGAP1
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RANGAP1
P46060 NM_002883 NP_002874.1

Validation

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Requested From: United States
Date Requested: 9/23/2019