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| Catalog Number | Size | Price |
|---|---|---|
| LS-B7373-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Polyclonal Goat anti‑Human RAMA1 / SKA3 Antibody (aa311‑323, IHC) LS‑B7373
IHC‑plus™ Polyclonal Goat anti‑Human RAMA1 / SKA3 Antibody (aa311‑323, IHC) LS‑B7373
Note: This antibody replaces LS-C112839
Antibody:
RAMA1 / SKA3 Goat anti-Human Polyclonal (aa311-323) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Dog
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
RAMA1 / SKA3 Goat anti-Human Polyclonal (aa311-323) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Dog
Format:
Unconjugated, Unmodified
Specifications
Description
SKA3 antibody LS-B7373 is an unconjugated goat polyclonal antibody to SKA3 (RAMA1) (aa311-323) from human. It is reactive with human and dog. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Host
Goat
Reactivity
Human, Dog
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-PLSKTNSSSNDLE, from the internal region of the protein sequence according to NP_659498.3.
Epitope
aa311-323
Specificity
Human SKA3.
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 35kDa and 150kDa band in HeLa lysates after 2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 46.4kDa according to NP_659498.3. Both bands were successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About RAMA1 / SKA3
Validation
Anti-SKA3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-SKA3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ RAMA1 / SKA3 Antibody (aa311-323) for IHC LS-B7373 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Reactivity:
Mouse
Range:
78-5000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
