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Antibodies > RAB3A > LS-B14834
 
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Catalog Number Size Price
LS-B14834-100 100 µl $460 
RAB3A Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAB3A Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAB3A Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal phaeochromocytoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on PC-12 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
RAB3A Antibody - Anti-RAB3A Antibody at 1:500 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RAB3A Antibody - All lanes: Anti-RAB3A Antibody at 1:2000 dilution. Lane 1: mouse brain lysate. Lane 2: rat brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RAB3A Antibody - Overlay histogram showing PC-12 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
RAB3A Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAB3A Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAB3A Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal phaeochromocytoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on PC-12 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
RAB3A Antibody - Anti-RAB3A Antibody at 1:500 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RAB3A Antibody - All lanes: Anti-RAB3A Antibody at 1:2000 dilution. Lane 1: mouse brain lysate. Lane 2: rat brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RAB3A Antibody - Overlay histogram showing PC-12 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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IHC‑plus™ Monoclonal Mouse anti‑Human RAB3A Antibody (clone 1531CT562.14.57, IHC, IF, WB) LS‑B14834

IHC‑plus™ Monoclonal Mouse anti‑Human RAB3A Antibody (clone 1531CT562.14.57, IHC, IF, WB) LS‑B14834

IHC Validated
(4.0) Rating
Submit Review LSBio Guarantee
Note: This antibody replaces LS-C392563
Antibody:
RAB3A Mouse anti-Human Monoclonal (1531CT562.14.57) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14834-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RAB3A Mouse anti-Human Monoclonal (1531CT562.14.57) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
RAB3A antibody LS-B14834 is an unconjugated mouse monoclonal antibody to RAB3A from human. It is reactive with human, mouse and rat. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RAB3A
Synonyms
RAB3A | RAS-associated protein RAB3A | Ras-related protein Rab-3A
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1531CT562.14.57
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:25)
  • Western blot (1:500)
  • Flow Cytometry (1:25)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAB3A
P20336 NM_002866 NP_002857.1

Validation

RAB3A Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
RAB3A Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ RAB3A Antibody (clone 1531CT562.14.57) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B14834 has an LSBio Rating of
(4.0) Rating
Laboratory Validation Score (4)

Publications (0)

Submit a Publication for LS-B14834

Customer Reviews (0)

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Species: Human, Monkey
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RAB3A Antibody - 1:100 staining human thyroid carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Reactivity: Porcine
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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