Human RAB3A

Rabbit

Human, Monkey (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

A 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (aa 202-217) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

aa202-217

Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (1 - 2 µg/ml)

Immunohistochemistry: LS-B3406 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3406 was determined to be 10 ug/ml.

Borate buffered saline, pH 8.2 (25mM sodium borate, 100mM boric acid, 75mM NaCl, 5mM EDTA (disodium salt)

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P20336 NCBI: NM_002866 NP_002857.1