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Immunohistochemistry Services

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B10589-50 50 µg (1 mg/ml) $425 
Proliferation Marker IPO-38 Antibody - Anti-Proliferation Marker IPO-38 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ Proliferation Marker IPO‑38 Antibody Monoclonal ELISA,IHC,IP,WB LS‑B10589

IHCPlus™ Proliferation Marker IPO‑38 Antibody Monoclonal ELISA,IHC,IP,WB LS‑B10589

Note: This antibody replaces LS-C59515
Antibody:
Mouse Monoclonal to Human Proliferation Marker IPO-38
Application:
IHC, IHC-P, IHC-Fr, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10589-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Proliferation Marker IPO-38
Application:
IHC, IHC-P, IHC-Fr, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Proliferation Marker IPO-38 antibody LS-B10589 is an unconjugated mouse monoclonal antibody to human Proliferation Marker IPO-38. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Proliferation Marker IPO-38
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Clone
IPO-38
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Spleen cells of a human patient with hairy cell leukemia.
Specificity
A 14 -16 kD protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.
Usage
This antibody is suitable for ELISA, immunoprecipitation, western blotting and immunohistochemistry (frozen and formal in/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-1 00 permeabilized). An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. Tonsilar tissue is typically used as a positive control.
Presentation
0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
Aliquot and store at -20°C or below for extended storage. Avoid freeze / thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Proliferation Marker IPO-38 Antibody - Anti-Proliferation Marker IPO-38 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Proliferation Marker IPO-38 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Proliferation Marker IPO-38 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 1/21/2020