Human PRMT5

Rabbit

Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

Synthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 100 of human Skb1Hs methyltransferase.

aa100

Recognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not cross-react with other related mammalian methyltransferases. Species cross-reactivity: monkey, mouse and rat.

• IHC
• IHC - Paraffin (1:100)
• Western blot (1:1000)
• Immunoprecipitation (1:100)

Immunohistochemistry: LS-B2433 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2433 was determined to be 1:100.

10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O14744 NCBI: NM_006109 NP_006100.2