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IHCPlus™ PPP1R12A / MYPT1 Antibody Polyclonal IF,IHC,Peptide‑ELISA LS‑B3836

Catalog Number Size Price
LS-B3836-50 50 µg (0.5 mg/ml) $425 
Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PPP1R12A / MYPT1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
PPP1R12A / MYPT1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PPP1R12A / MYPT1 antibody flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PPP1R12A / MYPT1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
PPP1R12A / MYPT1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PPP1R12A / MYPT1 antibody flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHCPlus™ PPP1R12A / MYPT1 Antibody Polyclonal IF,IHC,Peptide‑ELISA LS‑B3836

Note: This antibody replaces LS-C108241
Antibody:
Goat Polyclonal to Human PPP1R12A / MYPT1
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3836-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human PPP1R12A / MYPT1
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
MYPT1 antibody LS-B3836 is an unconjugated goat polyclonal antibody to MYPT1 (PPP1R12A ) from human, mouse, rat and other species. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human PPP1R12A / MYPT1
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-REDEYKQKYSRTYD, from the C Terminus of the protein sequence according to NP_002471.1, NP_001231919.1, NP_001231921 .1, NP_001137358.1.
Blocking Peptide
LS-E46678 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3836. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
Human PPP1R12A / MYPT1.
Usage
Immunohistochemistry: LS-B3836 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3836 was determined to be 3.75ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPP1R12A / MYPT1
O14974 NM_002480 NP_002471.1

Validation

Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
IHC Validated
Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

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Requested From: United States
Date Requested: 9/16/2019