(tested or 100% immunogen sequence identity)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.2 - 0.6 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Peptide with sequence C-EKPRARELRIS, from the internal region of the protein sequence according to NP_000296.2NP_001018171.1.
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8315. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human PON2. This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2; NP_001018171.1).
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 40kDa double band observed in lysates of HepG2 (calculated MW of 39.4kDa according to NP_000296.2 and of 38.0kDa according to NP_001018171.1). Recommended concentration: 0.2-0.6 ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.