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IHCPlus™ PNKP Antibody Internal Polyclonal ELISA,IF,IHC,WB LS‑B404

Catalog Number Size Price
LS-B404-50 50 µg (1 mg/ml) $425 
Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PNK Antibody - Western Blot. Western blot of Affinity Purified anti-PNK antibody shows detection of a 57 kD band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.
Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PNK Antibody - Western Blot. Western blot of Affinity Purified anti-PNK antibody shows detection of a 57 kD band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.
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IHCPlus™ PNKP Antibody Internal Polyclonal ELISA,IF,IHC,WB LS‑B404

Note: This antibody replaces LS-C18998
Antibody:
Rabbit Polyclonal to Human PNKP
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B404-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PNKP
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PNKP antibody LS-B404 is an unconjugated rabbit polyclonal antibody to human PNKP. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PNKP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1:300 - 1:1500)
  • ELISA (1:3000 - 1:12000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human PNKP.
Epitope
Internal
Specificity
An internal region of human PNK protein.
Usage
Immunohistochemistry: LS-B404 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B404 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PNKP
Q96T60 NM_007254 NP_009185.2

Validation

Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/16/2019