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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B404-50 50 µg (1 mg/ml) $425 
PNKP Antibody - Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PNKP Antibody - Anti-PNK Antibody - Western Blot. Western blot of Affinity Purified anti-PNK antibody shows detection of a 57 kD band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.
PNKP Antibody - Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PNKP Antibody - Anti-PNK Antibody - Western Blot. Western blot of Affinity Purified anti-PNK antibody shows detection of a 57 kD band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.
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IHCPlus™ Polyclonal Rabbit anti‑Human PNKP Antibody (IHC, WB) LS‑B404

IHCPlus™ Polyclonal Rabbit anti‑Human PNKP Antibody (IHC, WB) LS‑B404

Note: This antibody replaces LS-C18998
Antibody:
Rabbit Polyclonal to Human PNKP
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B404-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human PNKP
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified

Specifications

Description
PNKP antibody LS-B404 is an unconjugated rabbit polyclonal antibody to PNKP from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PNKP
Synonyms
PNKP | EIEE10 | DNA 5-kinase/3-phosphatase | PNK | MCSZ
Host
Rabbit
Reactivity
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:300 - 1:1500)
  • ELISA (1:3000 - 1:12000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PNK protein.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Expect cross-reactivity with PNK from dog, mouse and rat sources, as only two amino acid residues change within the immunogen sequence (83% positive by BLAST). Cross-reactivity with PNK homologues from other sources has not been determined.
Usage
Immunohistochemistry: LS-B404 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B404 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PNKP
Q96T60 NM_007254 NP_009185.2

Validation

PNKP Antibody - Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ PNKP Antibody for IHC, WB/Western, ELISA LS-B404 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/13/2020