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Catalog Number Size Price
LS-B13361-50 50 µl $460 
PML Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
PML Antibody - Immunofluorescence analysis of HeLa cells using PML antibody. Blue: DAPI for nuclear staining.
PML Antibody - Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
PML Antibody - Western blot analysis of extracts of various cell lines, using PML antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PML Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
PML Antibody - Immunofluorescence analysis of HeLa cells using PML antibody. Blue: DAPI for nuclear staining.
PML Antibody - Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
PML Antibody - Western blot analysis of extracts of various cell lines, using PML antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PML Antibody (IHC, IF, WB) LS‑B13361

IHC‑plus™ Polyclonal Rabbit anti‑Human PML Antibody (IHC, IF, WB) LS‑B13361

Note: This antibody replaces LS-C331332
Antibody:
PML Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B13361-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PML Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PML antibody LS-B13361 is an unconjugated rabbit polyclonal antibody to PML from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PML
Synonyms
PML | PP8675 | Promyelocytic leukemia | Protein PML | TRIM19 | Promyelocytic leukemia protein | RING finger protein 71 | MYL | RNF71
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2). AVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSCMEPMETAEPQSSPAHSSPAHSSPAHSSPVQSLLRAQGASSLPCGTYHPPAWPPHQPAEQAATPDAEPHSEPPDHQER
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 47-48kDa/62-97kDa, while the observed MW by Western blot was 98kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PML
P29590 NM_002675 NP_002666.1

Validation

PML Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ PML Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B13361 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/30/2021