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Catalog Number Size Price
LS-B11910-50 50 µl $460 
PKM / Pyruvate Kinase, Muscle Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
PKM / Pyruvate Kinase, Muscle Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
PKM / Pyruvate Kinase, Muscle Antibody - Immunohistochemistry of paraffin-embedded rat kidney using PKM2 antibody at dilution of 1:100 (200x lens).
PKM / Pyruvate Kinase, Muscle Antibody - Immunohistochemistry of paraffin-embedded mouse lung using PKM2 antibody at dilution of 1:100 (400x lens).
PKM / Pyruvate Kinase, Muscle Antibody - Immunofluorescence analysis of U2OS cells using PKM antibody. Blue: DAPI for nuclear staining.
PKM / Pyruvate Kinase, Muscle Antibody - Immunofluorescence analysis of HeLa cell using PKM2 antibody. Blue: DAPI for nuclear staining.
PKM / Pyruvate Kinase, Muscle Antibody - Western blot analysis of extracts of various cell lines, using PKM antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PKM / Pyruvate Kinase, Muscle Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
PKM / Pyruvate Kinase, Muscle Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
PKM / Pyruvate Kinase, Muscle Antibody - Immunohistochemistry of paraffin-embedded rat kidney using PKM2 antibody at dilution of 1:100 (200x lens).
PKM / Pyruvate Kinase, Muscle Antibody - Immunohistochemistry of paraffin-embedded mouse lung using PKM2 antibody at dilution of 1:100 (400x lens).
PKM / Pyruvate Kinase, Muscle Antibody - Immunofluorescence analysis of U2OS cells using PKM antibody. Blue: DAPI for nuclear staining.
PKM / Pyruvate Kinase, Muscle Antibody - Immunofluorescence analysis of HeLa cell using PKM2 antibody. Blue: DAPI for nuclear staining.
PKM / Pyruvate Kinase, Muscle Antibody - Western blot analysis of extracts of various cell lines, using PKM antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PKM / Pyruvate Kinase, Muscle Antibody (IHC, IF, WB) LS‑B11910

IHC‑plus™ Polyclonal Rabbit anti‑Human PKM / Pyruvate Kinase, Muscle Antibody (IHC, IF, WB) LS‑B11910

Note: This antibody replaces LS-C335088
Antibody:
PKM / Pyruvate Kinase, Muscle Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP, RnaIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B11910-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PKM / Pyruvate Kinase, Muscle Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP, RnaIP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Pyruvate Kinase, Muscle antibody LS-B11910 is an unconjugated rabbit polyclonal antibody to human Pyruvate Kinase, Muscle (PKM). Validated for IF, IHC, IP, RnaIP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PKM / Pyruvate Kinase, Muscle
Synonyms
PKM | p58 | PK3 | PKM2 | Pyruvate Kinase | Pyruvate kinase 2/3 | Pyruvate kinase, m1 | Pyruvate kinase, muscle | TCB | Tumor M2-PK | OIP-3 | OIP3 | Opa-interacting protein 3 | CTHBP | PK, muscle type | PK2 | Pyruvate kinase isozymes M1/M2 | Pyruvate kinase muscle isozyme | THBP1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 11-221 of human PKM (NP_002645.3). AFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAV
Specificity
Human PKM / Pyruvate Kinasex
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:20 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:20 - 1:50)
  • RNA Immunoprecipitation (1:20 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 56kDa/57kDa/58kDa, while the observed MW by Western blot was 62kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PKM / Pyruvate Kinase, Muscle
P14618 NM_002654 NP_002645.3

Validation

PKM / Pyruvate Kinase, Muscle Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

PKM / Pyruvate Kinase, Muscle Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ PKM / Pyruvate Kinase, Muscle Antibody for IHC, IF/Immunofluorescence, WB/Western, IP LS-B11910 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024