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Catalog Number Size Price
LS-B10608-50 50 µg (1.1 mg/ml) $485 
PIN1 Antibody - Anti-PIN1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PIN1 Antibody - Anti-Pin1 Antibody - Western Blot. Western blot of affinity purified anti-Pin1 antibody to detect endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5000 dilution of HRP Gt-a-Rabbit IgG (LS-C60865) was used with a 15 sec exposure time. Personal Communication, L. D'Agostino and A. Giordano, SHRO, Philadelphia, PA.
PIN1 Antibody - Anti-PIN1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PIN1 Antibody - Anti-Pin1 Antibody - Western Blot. Western blot of affinity purified anti-Pin1 antibody to detect endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5000 dilution of HRP Gt-a-Rabbit IgG (LS-C60865) was used with a 15 sec exposure time. Personal Communication, L. D'Agostino and A. Giordano, SHRO, Philadelphia, PA.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PIN1 Antibody (IHC, WB) LS‑B10608

IHC‑plus™ Polyclonal Rabbit anti‑Human PIN1 Antibody (IHC, WB) LS‑B10608

Note: This antibody replaces LS-C60112
Antibody:
PIN1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Monkey, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B10608-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PIN1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Monkey, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
PIN1 antibody LS-B10608 is an unconjugated rabbit polyclonal antibody to PIN1 from human. It is reactive with human, bovine, dog and other species. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PIN1
Synonyms
PIN1 | DOD | PPIase Pin1 | Rotamase Pin1
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Dog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Pin1.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Pin1 from human, dog, bovine and monkey based on a 100% homology with the immunizing sequence. Expect partial reactivity with Pin1 from mouse and rat sources based on 92% sequence homologies. Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation (1:100)
  • ELISA (1:2500 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kD in size corresponding to Pin1 by western blotting in the appropriate cell lysate or extract. Lysates from 3T3, Jurkat, 293 or HeLa cells, as well as HeLa nuclear extract, are recommended for use as positive controls.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PIN1
Q13526 NM_006221 NP_006212.1

Validation

PIN1 Antibody - Anti-PIN1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PIN1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PIN1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ PIN1 Antibody for IHC, WB/Western, IP, ELISA LS-B10608 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/4/2022