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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-B5809-100 100 µl $485 
PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PGK1 / Phosphoglycerate Kinase Antibody - Hela cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PGK1 / Phosphoglycerate Kinase Antibody - Hela cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ Polyclonal Rabbit anti‑Human PGK1 / Phosphoglycerate Kinase Antibody (aa102‑151, IHC, WB) LS‑B5809

IHCPlus™ Polyclonal Rabbit anti‑Human PGK1 / Phosphoglycerate Kinase Antibody (aa102‑151, IHC, WB) LS‑B5809

Note: This antibody replaces LS-C81162
Antibody:
Rabbit Polyclonal to Human PGK1 / Phosphoglycerate Kinase
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5809-100
100 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human PGK1 / Phosphoglycerate Kinase
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Phosphoglycerate Kinase antibody LS-B5809 is an unconjugated rabbit polyclonal antibody to Phosphoglycerate Kinase (PGK1) (aa102-151) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human PGK1 / Phosphoglycerate Kinase
Synonyms
PGK1 | MIG10 | Primer recognition protein 2 | PRP 2 | PGKA | Phosphoglycerate kinase 1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa102-151 of human PGK1 (P00558, NP_000282). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%); Hamster, Elephant, Pig, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Salmon, Stickleback, Zebrafish, Mosquito (92%); Medaka, Drosophila (85%).
Blocking Peptide
LS-E17410 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5809. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa102-151
Specificity
Human PGK1
Usage
Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PGK1 / Phosphoglycerate Kinase
P00558 NM_000291 NP_000282.1

Validation

PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ PGK1 / Phosphoglycerate Kinase Antibody (aa102-151) for IHC, WB/Western LS-B5809 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Ekman M, Uvelius B, Albinsson S, Sw[Character e4]rd K. Laboratory investigation; a journal of technical methods and pathology. May;94:557-68.

Mitochondrial impairment in the five-sixth nephrectomy model of chronic renal failure: proteomic approach. Fedorova LV, Tamirisa A, Kennedy DJ, Haller ST, Budnyy G, Shapiro JI, Malhotra D. BMC nephrology. 2013 14:209. (WB; Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 6/2/2020