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IHCPlus™ PGK1/Phosphoglycerate Kinase 1 Antibody aa102‑151 Polyclonal IHC,WB LS‑B5809

Catalog Number Size Price
LS-B5809-100 100 µl $485 
Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ PGK1/Phosphoglycerate Kinase 1 Antibody aa102‑151 Polyclonal IHC,WB LS‑B5809

Note: This antibody replaces LS-C81162
Antibody:
Rabbit Polyclonal to Human PGK1/Phosphoglycerate Kinase 1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5809-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PGK1/Phosphoglycerate Kinase 1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Phosphoglycerate Kinase 1 antibody LS-B5809 is an unconjugated rabbit polyclonal antibody to Phosphoglycerate Kinase 1 (PGK1) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human PGK1/Phosphoglycerate Kinase 1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa102-151 of human PGK1 (P00558, NP_000282). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%); Hamster, Elephant, Pig, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Salmon, Stickleback, Zebrafish, Mosquito (92%); Medaka, Drosophila (85%).
Blocking Peptide
LS-E17410 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5809. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa102-151
Specificity
Human PGK1
Usage
Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PGK1/Phosphoglycerate Kinase 1
P00558 NM_000291 NP_000282.1

Validation

Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (2)

HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Ekman M, Uvelius B, Albinsson S, Sw[Character e4]rd K. Laboratory investigation; a journal of technical methods and pathology. May;94:557-68.

Mitochondrial impairment in the five-sixth nephrectomy model of chronic renal failure: proteomic approach. Fedorova LV, Tamirisa A, Kennedy DJ, Haller ST, Budnyy G, Shapiro JI, Malhotra D. BMC nephrology. 2013 14:209. (WB; Human)

Reviews (0)

Featured Products

Anti-PGK1/Phosphoglycerate Kinase 1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PGK1 antibody PGK1(phosphoglycerate kinase 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bat, Bovine, Dog, Rabbit, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry
Immunofluorescent analysis of PGK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
Knockout Varification: Lane 1: Wild-type Hela cell lysate; Lane 2: PGK1 knockout Hela cell lysate; Predicted MW: 45kDa ; Observed MW: 45kDa; Primary Ab: 1µg/ml Rabbit Anti-Human PGK1 Antibody; Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
Species: Human, Pig
Applications: ICC, Western blot, Immunoprecipitation

Requested From: United States
Date Requested: 8/25/2019
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