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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3459-100 | 100 µl | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human PDK4 Antibody (aa382‑410, IHC, IF, WB) LS‑B3459
IHC‑plus™ Polyclonal Rabbit anti‑Human PDK4 Antibody (aa382‑410, IHC, IF, WB) LS‑B3459
Note: This antibody replaces LS-C98959
Antibody:
PDK4 Rabbit anti-Human Polyclonal (aa382-410) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PDK4 Rabbit anti-Human Polyclonal (aa382-410) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
PDK4 antibody LS-B3459 is an unconjugated rabbit polyclonal antibody to PDK4 (aa382-410) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa382-410
Specificity
This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Immunofluorescence (1:100)
- Western blot (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3459 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use with mouse in IHC-P.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PDK4
Validation
Anti-PDK4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-PDK4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-PDK4 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-PDK4 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ PDK4 Antibody (aa382-410) for IHC, IF/Immunofluorescence, WB/Western LS-B3459 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (4)
Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression. Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA. The Journal of biological chemistry. 2006 281:39897-906. (WB, ChrIP; Rat)
Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by thyroid hormone: role of the peroxisome proliferator-activated receptor gamma coactivator (PGC-1 alpha). Attia RR, Connnaughton S, Boone LR, Wang F, Elam MB, Ness GC, Cook GA, Park EA. The Journal of biological chemistry. 2010 285:2375-85.
Coenzyme Q10 reverses mitochondrial dysfunction in atorvastatin-treated mice and increases exercise endurance. Muraki A, Miyashita K, Mitsuishi M, Tamaki M, Tanaka K, Itoh H. Journal of applied physiology (Bethesda, Md. : 1985). 2013 Aug;113:479-86.
MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells. Mazar J, Qi F, Lee B, Marchica J, Govindarajan S, Shelley J, Li JL, Ray A, Perera RJ. Molecular and cellular biology. 2016 36:1090-108. (WB; Human)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
