Synthetic peptide located between aa216-265 of human ALKBH3 (Q96Q83, NP_631917). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bat, Horse, Smelt (100%); Dog, Bovine, Guinea pig, Chicken, Platypus, Xenopus, Stickleback (92%); Rabbit, Turkey, Catfish (85%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
This antibody carries the LSBio 100% Guarantee.