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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B5865-100 100 µl $485 
PCA1 / ALKBH3 Antibody - Anti-ALKBH3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PCA1 / ALKBH3 Antibody - ALKBH3 antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PCA1 / ALKBH3 Antibody - Anti-ALKBH3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PCA1 / ALKBH3 Antibody - ALKBH3 antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ Polyclonal PCA1 / ALKBH3 Antibody (aa216‑265, IHC, WB) LS‑B5865

IHCPlus™ Polyclonal PCA1 / ALKBH3 Antibody (aa216‑265, IHC, WB) LS‑B5865

Note: This antibody replaces LS-C102638
Antibody:
Rabbit Polyclonal to Human PCA1 / ALKBH3
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5865-100
100 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PCA1 / ALKBH3
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
ALKBH3 antibody LS-B5865 is an unconjugated rabbit polyclonal antibody to ALKBH3 (PCA1 ) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PCA1 / ALKBH3
Synonyms
ALKBH3 | ABH3 | DEPC1 | DEPC-1 | Prostate cancer antigen 1 | PCA1 | Prostate cancer antigen-1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa216-265 of human ALKBH3 (Q96Q83, NP_631917). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bat, Horse, Smelt (100%); Dog, Bovine, Guinea pig, Chicken, Platypus, Xenopus, Stickleback (92%); Rabbit, Turkey, Catfish (85%).
Blocking Peptide
LS-E20031 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5865. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa216-265
Specificity
Human ALKBH3
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PCA1 / ALKBH3
Q96Q83 Q96Q83

Validation

PCA1 / ALKBH3 Antibody - Anti-ALKBH3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ALKBH3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ALKBH3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 2/22/2020