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IHCPlus™ PAX6 Antibody aa32‑81 Polyclonal IHC,WB LS‑B991

Catalog Number Size Price
LS-B991-100 100 µl $485 
Anti-PAX6 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PAX6 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PAX6 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PAX6 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ PAX6 Antibody aa32‑81 Polyclonal IHC,WB LS‑B991

Note: This antibody replaces LS-C30197
Antibody:
Rabbit Polyclonal to Human PAX6
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rat, Bovine, Dog, Horse, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B991-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PAX6
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rat, Bovine, Dog, Horse, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
PAX6 antibody LS-B991 is an unconjugated rabbit polyclonal antibody to PAX6 from human, rat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PAX6
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bovine, Dog, Horse, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.06 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa32-81 of human PAX6 (P26367-2, NP_001595). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine, Rabbit, Horse, Opossum, Zebra finch, Chicken, Platypus, Lizard (100%); Mouse, Guinea pig, Newt, Zebrafish (92%); Rat (85%); Xenopus (84%).
Blocking Peptide
LS-E13652 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B991. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa32-81
Specificity
Human PAX6
Usage
Immunohistochemistry: LS-B991 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B991 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PAX6
P26367 NM_001604 NP_001595.2

Validation

Anti-PAX6 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-PAX6 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PAX6 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 9/17/2019