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Catalog Number Size Price
LS-B9556-50 50 µg (0.5 mg/ml) $485 
P2RX4 / P2X4 Antibody - Anti-P2RX4 / P2X4 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
P2RX4 / P2X4 Antibody - P2RX4 / P2X4 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
P2RX4 / P2X4 Antibody - P2RX4 / P2X4 antibody (0.3µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
P2RX4 / P2X4 Antibody - P2RX4 antibody (1 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
P2RX4 / P2X4 Antibody - Anti-P2RX4 / P2X4 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
P2RX4 / P2X4 Antibody - P2RX4 / P2X4 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
P2RX4 / P2X4 Antibody - P2RX4 / P2X4 antibody (0.3µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
P2RX4 / P2X4 Antibody - P2RX4 antibody (1 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Mouse P2RX4 / P2X4 Antibody (aa56‑69, IHC, IF, WB) LS‑B9556

IHC‑plus™ Polyclonal Goat anti‑Mouse P2RX4 / P2X4 Antibody (aa56‑69, IHC, IF, WB) LS‑B9556

Note: This antibody replaces LS-C154979
Antibody:
P2RX4 / P2X4 Goat anti-Mouse Polyclonal (aa56-69) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Mouse, Human, Rat, Dog, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B9556-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
P2RX4 / P2X4 Goat anti-Mouse Polyclonal (aa56-69) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Mouse, Human, Rat, Dog, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
P2X4 antibody LS-B9556 is an unconjugated goat polyclonal antibody to P2X4 (P2RX4) (aa56-69) from mouse. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Mouse P2RX4 / P2X4
Synonyms
P2RX4 | ATP receptor | p2X purinoceptor 4 | p2X4R | p2RX4 | p2X receptor 4 | p2X4 | p2X4 purinoceptor | Purinergic receptor P2X4 | Purinoceptor P2X4 | Purinergic receptor | p2X receptor, subunit 4
Host
Goat
Reactivity
Mouse, Human, Rat, Dog, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-ETDSVVSSVTTKAK, from the internal region of the protein sequence according to NP_035156.2.
Epitope
aa56-69
Specificity
Mouse P2RX4 / P2X4.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 60kD band observed in Human Kidney and Liver lysates (calculated MW of 43.5kD according to NP_002551.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Brown et al, J Biol Chem. 2004 Sep 17;279(38):39485-94. PMID: 15262999). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About P2RX4 / P2X4
Q99571 NM_002560 NP_002551.2

Validation

P2RX4 / P2X4 Antibody - Anti-P2RX4 / P2X4 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-P2RX4 / P2X4 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-P2RX4 / P2X4 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ P2RX4 / P2X4 Antibody (aa56-69) for IHC, IF/Immunofluorescence, WB/Western LS-B9556 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024