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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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IHCPlus™ OCLN / Occludin Antibody aa35‑84 Polyclonal IHC,WB LS‑B5737

Catalog Number Size Price
LS-B5737-100 100 µl (0.5 mg/ml) $485 
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
OCLN / Occludin antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
OCLN / Occludin antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ OCLN / Occludin Antibody aa35‑84 Polyclonal IHC,WB LS‑B5737

Note: This antibody replaces LS-C110724
Antibody:
Rabbit Polyclonal to Human OCLN / Occludin
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5737-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human OCLN / Occludin
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Occludin antibody LS-B5737 is an unconjugated rabbit polyclonal antibody to Occludin (OCLN ) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human OCLN / Occludin
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa35-84 of human OCLN (Q16625, NP_002529). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Opossum, Platypus, Xenopus (92%); Bat (85%); Trout, Zebrafish (81%).
Blocking Peptide
LS-E15782 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5737. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human OCLN / Occludin
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About OCLN / Occludin
Q16625 NM_002538 NP_002529.1

Validation

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 8/23/2019
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