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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B6270-50 50 µl (1 mg/ml) $425 
NRAS / N-ras Antibody - Anti-NRAS antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
NRAS / N-ras Antibody - Extracts of 293T(35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS(AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS.
NRAS / N-ras Antibody - Anti-NRAS antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
NRAS / N-ras Antibody - Extracts of 293T(35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS(AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS.
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IHC‑plus™ Monoclonal Mouse anti‑Human NRAS / N‑ras Antibody (clone AT2G9, IHC, WB) LS‑B6270

IHC‑plus™ Monoclonal Mouse anti‑Human NRAS / N‑ras Antibody (clone AT2G9, IHC, WB) LS‑B6270

Note: This antibody replaces LS-C139556
Antibody:
NRAS / N-ras Mouse anti-Human Monoclonal (AT2G9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B6270-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
NRAS / N-ras Mouse anti-Human Monoclonal (AT2G9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
N-ras antibody LS-B6270 is an unconjugated mouse monoclonal antibody to human N-ras (NRAS). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NRAS / N-ras
Synonyms
NRAS | HRAS1 | NRAS1 | NS6 | ALPS4 | Transforming protein N-Ras | N-ras | N-ras protein part 4 | GTPase NRas
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
AT2G9
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant protein
Specificity
Human NRAS
Applications
  • IHC
  • IHC - Paraffin (15 µg/ml)
  • Western blot (1:500 - 1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NRAS / N-ras
P01111 NM_002524 NP_002515.1

Validation

NRAS / N-ras Antibody - Anti-NRAS antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Anti-NRAS antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Anti-NRAS antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.

LSBio Ratings

IHC-plus™ NRAS / N-ras Antibody (clone AT2G9) for IHC, WB/Western, ELISA LS-B6270 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/2/2020