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IHCPlus™ NR5A2 / LRH‑1 Antibody Internal Polyclonal FITC IHC LS‑A102842

Catalog Number Size Price
LS-A102842-100 100 µl $650 
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

IHCPlus™ NR5A2 / LRH‑1 Antibody Internal Polyclonal FITC IHC LS‑A102842

Antibody:
Rabbit Polyclonal to Human NR5A2 / LRH-1
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Chicken, Xenopus, Zebrafish
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$650 
LS-A102842-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NR5A2 / LRH-1
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Chicken, Xenopus, Zebrafish
Format:
FITC, Unmodified
Other formats:

Specifications

Description
LRH-1 antibody LS-A102842 is an FITC-conjugated rabbit polyclonal antibody to LRH-1 (NR5A2 ) from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human NR5A2 / LRH-1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
FITC. Also available Unconjugated or conjugated with AP, APC, Biotin, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 16 amino acid peptide from internal region of human NR5A2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Elephant, Panda, Horse, Opossum, Turkey, Chicken, Platypus, Xenopus, Trout, Zebrafish, Stickleback (100%); Sheep, Bovine, Bat, Pufferfish (94%); Chimpanzee (88%); Catfish (81%).
Epitope
Internal
Specificity
Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A2453 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2453 was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NR5A2 / LRH-1
O00482 NM_003822 NP_003813.1

Validation

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Publications (0)

Reviews (0)

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Requested From: United States
Date Requested: 8/24/2019
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