Synthetic 16 amino acid peptide from internal region of human NR5A2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Elephant, Panda, Horse, Opossum, Turkey, Chicken, Platypus, Xenopus, Trout, Zebrafish, Stickleback (100%); Sheep, Bovine, Bat, Pufferfish (94%); Chimpanzee (88%); Catfish (81%).
Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2453
was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.