Human NR1I2 / PXR

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence EQFAITLKSYIECNR, from the internal region of the protein sequence according to NP_003880.3NP_071285.1NP_148934.1.

aa406-420

Human NR1I2 / PXR. This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).

• IHC
• IHC - Paraffin (3.75 µg/ml)
• Western blot (1 - 3 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:4000)

Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx. 48kD band observed in lysates of cell line HepG2 (calculated MW of 49.8kD according to NP_003880.3). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 35kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O75469 NCBI: NM_003889 NP_003880.3