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Catalog Number Size Price
LS-B2582-50 50 µg (1 mg/ml) $460 
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC‑plus™ Monoclonal Rat anti‑Human NFKB1 / NF‑Kappa‑B Antibody (clone NF5E6, aa1‑15, IHC, WB) LS‑B2582

IHC‑plus™ Monoclonal Rat anti‑Human NFKB1 / NF‑Kappa‑B Antibody (clone NF5E6, aa1‑15, IHC, WB) LS‑B2582

Note: This antibody replaces LS-C9275
Antibody:
NFKB1 / NF-Kappa-B Rat anti-Human Monoclonal (aa1-15) (NF5E6) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2582-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
NFKB1 / NF-Kappa-B Rat anti-Human Monoclonal (aa1-15) (NF5E6) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NF-Kappa-B antibody LS-B2582 is an unconjugated rat monoclonal antibody to human NF-Kappa-B (NFKB1) (aa1-15). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NFKB1 / NF-Kappa-B
Synonyms
NFKB1 | Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
NF5E6
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant human NF- B (p50 subunit).
Epitope
aa1-15
Specificity
Recognizes p50 subunit of human NF-kB (epitope in the region aa 1-15).
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2582 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2582 was determined to be 10 ug/ml.
Presentation
Tris-Glycine, 0.05% Sodium Azide
Storage
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKB1 / NF-Kappa-B
P19838 NM_003998 NP_003989.2

Validation

NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NFKB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NFKB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ NFKB1 / NF-Kappa-B Antibody (aa1-15, clone NF5E6) for IHC, WB/Western LS-B2582 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/24/2022