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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2582-50 | 50 µg (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Rat anti‑Human NFKB1 / NF‑Kappa‑B Antibody (clone NF5E6, aa1‑15, IHC, WB) LS‑B2582
IHC‑plus™ Monoclonal Rat anti‑Human NFKB1 / NF‑Kappa‑B Antibody (clone NF5E6, aa1‑15, IHC, WB) LS‑B2582
Note: This antibody replaces LS-C9275
Antibody:
NFKB1 / NF-Kappa-B Rat anti-Human Monoclonal (aa1-15) (NF5E6) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NFKB1 / NF-Kappa-B Rat anti-Human Monoclonal (aa1-15) (NF5E6) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
NF-Kappa-B antibody LS-B2582 is an unconjugated rat monoclonal antibody to human NF-Kappa-B (NFKB1) (aa1-15). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NFKB1 / NF-Kappa-B
Synonyms
NFKB1 | Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta
Host
Rat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
NF5E6
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant human NF- B (p50 subunit).
Epitope
aa1-15
Specificity
Recognizes p50 subunit of human NF-kB (epitope in the region aa 1-15).
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2582 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2582 was determined to be 10 ug/ml.
Presentation
Tris-Glycine, 0.05% Sodium Azide
Storage
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NFKB1 / NF-Kappa-B
Validation
Anti-NFKB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NFKB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NFKB1 / NF-Kappa-B Antibody (aa1-15, clone NF5E6) for IHC, WB/Western LS-B2582 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
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Species:
Human, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
![NFKB1 / NF-Kappa-B Antibody - Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/nfkb1-nf-kappa-b-antibody-clone-3f6-ls-c133346/51309_1017279.jpg)
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
