Human NFATC1 / NFAT2

Rabbit

Human, Monkey (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein G purified

Unmodified

Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Mouse, Rat, Rabbit, Turkey, Chicken, Lizard (89%); Marmoset, Opossum, Xenopus (84%).

aa263-282

The amino acid sequence used is 90% homologous in mouse and rat and 100% identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (1 - 3 µg/ml)

Immunohistochemistry: LS-B1977 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1977 was determined to be 10 ug/ml.

PBS, 0.05% Sodium Azide, 0.05% BSA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95644 NCBI: NM_006162 NP_006153.2