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| Catalog Number | Size | Price |
|---|---|---|
| LS-B394-50 | 50 µg | $515 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human NF2 / Merlin Antibody (phospho‑Ser518, IHC, WB) LS‑B394
IHC‑plus™ Polyclonal Rabbit anti‑Human NF2 / Merlin Antibody (phospho‑Ser518, IHC, WB) LS‑B394
Note: This antibody replaces LS-C18844, LS-C72427
Antibody:
NF2 / Merlin Rabbit anti-Human Polyclonal (pSer518) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NF2 / Merlin Rabbit anti-Human Polyclonal (pSer518) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
Merlin antibody LS-B394 is an unconjugated rabbit polyclonal antibody to Merlin (NF2) (pSer518) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NF2 / Merlin
Synonyms
NF2 | ACN | BANF | Moesin-ezrin-radixin like | Neurofibromin 2 (merlin) | Neurofibromin-2 | SCH | Merlin | Schwannomerlin | Schwannomin
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
pSer518
Specificity
This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross-reactivity with NF2 (Merlin) occurs in mouse tissue. Reactivity with NF2 from other sources has not been determined.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA (1:1200000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B394 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B394 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NF2 / Merlin
Validation
Anti-NF2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NF2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NF2 / Merlin Antibody (phospho-Ser518) for IHC, WB/Western, ELISA LS-B394 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
