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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ NES / Nestin Antibody Monoclonal IHC,WB LS‑B7877

Catalog Number Size Price
LS-B7877-50 50 µg (0.5 mg/ml) $425 
Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
U251 cell lysates prepared under non-reducing (lane 1) or reducing (lane 2) conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of anti-human nestin (10C2) antibody. Bands were visualized using HRP-conjugated anti-mouse IgG.
Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
U251 cell lysates prepared under non-reducing (lane 1) or reducing (lane 2) conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of anti-human nestin (10C2) antibody. Bands were visualized using HRP-conjugated anti-mouse IgG.
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IHCPlus™ NES / Nestin Antibody Monoclonal IHC,WB LS‑B7877

Note: This antibody replaces LS-C107085
Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7877-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nestin antibody LS-B7877 is an unconjugated mouse monoclonal antibody to human Nestin (NES ). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NES / Nestin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
10C2
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human NES
Usage
This 10C2 antibody has been tested by immunoblot analysis of cell line. This can be used at less than or equal to 5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NES / Nestin
P48681 X65964 CAA46780.1

Validation

Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
IHC Validated
Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.

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Requested From: United States
Date Requested: 8/23/2019
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