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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ NES / Nestin Antibody Monoclonal ICC,IHC,WB LS‑B7181

Catalog Number Size Price
LS-B7181-0.05 0.05 mg (1 mg/ml) $425 
Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunostaining of intracellular nestin filaments in U251 cells
Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells using NES / Nestin (1:100 dilution).
Immunoblotting of U251-cell lysate showing reactivity with nestin
Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunostaining of intracellular nestin filaments in U251 cells
Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells using NES / Nestin (1:100 dilution).
Immunoblotting of U251-cell lysate showing reactivity with nestin
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IHCPlus™ NES / Nestin Antibody Monoclonal ICC,IHC,WB LS‑B7181

Note: This antibody replaces LS-C146775
Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7181-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nestin antibody LS-B7181 is an unconjugated mouse monoclonal antibody to human Nestin (NES ). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NES / Nestin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1 Monoclonal
Clone
10C2
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:100 - 1:200)
  • ICC
  • Western blot (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 150-amino-acid fragment from the cloned human nestin was conjugated to glutathione S-transferase
Specificity
Reacts exclusively with the human nestin protein. No cross-reactivity to rodent nestin.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NES / Nestin
P48681 X65964 CAA46780.1

Validation

Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Nestin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Requested From: United States
Date Requested: 8/19/2019
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